引物分析设计软件 Oligo v6 v7

OLIGO引物分析软件是设计和分析测序和PCR引物、合成基因以及包括siRNA 和分子信标在内的各种探针的必备工具。

基于新的nearest neighbor热力学数据，Oligo的搜索算法可以找到PCR的optimal引物，包括TaqMan、高度多重、一致或简并的引物。可以进行多文件批处理。它也是定点诱变的宝贵工具。

对于每个引物或引物对，Oligo的各种分析窗口会显示大量有用的数据，例如 DNA和RNA二级结构、二聚体形成、假启动和同源性、内部稳定性、组成和物理特性。使用Oligo，您可以分析开放阅读框，细化到预测的分子量和蛋白质的pKa，并搜索限制酶位点，不只在DNA中，而且在反向翻译的蛋白质中。

该软件的初始版本于1989年面世，经历了数次修改，版本6到版本7的变化是为全面。Oligo 7可以自动选择多重引物，以批处理模式处理序列文件，自动设计PCR引物以在一次搜索中覆盖多个DNA区域，并自动为实时PCR寻找引物/探针组或寻找嵌套引物组。寡核苷酸搜索协议（评分系统）可能会进行详细定制，因此您可以根据自己的具体需求优化结果。

引物分析设计软件 Oligo v6 v7(图1)

OLIGO Primer Analysis Software is the essential tool for designing and analyzing sequencing and PCR primers, synthetic genes, and various kinds of probes including siRNA and molecular beacons.
Based on the most up-to date nearest neighbor thermodynamic data, Oligo’s search algorithms find optimal primers for PCR, including TaqMan, highly multiplexed, consensus or degenerate primers. Multiple file batch processing is possible. It is also an invaluable tool for site directed mutagenesis.
For each primer or primer pair, Oligo’s various analysis windows show a multitude of useful data, such as DNA and RNA secondary structure, dimer formation, false priming and homology, internal stability, composition and physical properties. With Oligo you can analyze open reading frames down to predicted molecular weight and pKa of proteins, and search for restriction enzyme sites, not only in DNA but also in reverse-translated proteins.
The first version of the software appeared on the market in 1989. It went through several modifications, and the last one, the change from version 6 to 7, was the most comprehensive. Oligo 7 can automatically select multiplex primers, process sequence files in batch modes, automatically design PCR primers to cover multiple DNA regions in just one search and automatically find primer/probe sets for real time PCR or finds nested primers sets. Oligo search protocols (scoring system) may be customized in detail, so you may optimize the results according to your specific needs.
Oligo runs on Macintosh and Windows and it may be downloaded from this site. Click on the Tutorials link above to see Oligo 7 general overview and the major windows screen designs.

OLIGO’s comprehensive functionality matches several other commercial primer design applications put together, so you can design probes for high throughput analysis, multiplex them or focus on one set of primers/probes and optimize it for a new, not yet published application.

Home Page: https://www.oligo.net/